Plant tissue culture methods and applications in agriculture f quak netherlands journal of plant pathology volume 88 page 215 1982cite this article 108 accesses 2 citations this is a preview of subscription content log in to check access authors search for f quak in pubmed o google scholar rights and permissions reprints and permissions about this article cite this . This book is the proceedings of a symposium based on the unesco training course on plant tissue culture methods and applications in agriculture sponsored by unesco and held at campinas sao paulo brazil on 8 22 nov 1978 it is divided into sections covering methodology 7 papers and applications of methods 7 papers 5 papers are abstracted separately in fca and hba and 2 in fcaltnew. Plant tissue culture methods and applications in agriculture contains the proceedings of a symposium based on the unesco training course on plant tissue culture methods and applications in agriculture sponsored by unesco and held in campinas sao paulo brazil on november 8 22 1978 this book contains two major sections encompassing plant tissue culture part a which focuses on methodology and part b which emphasizes the applications the first chapters present the requirements for a . Application of plant tissue culture in horticulture a clonal propagation the conventional method of clonal propagation are slow often not applicable for example the only in vivo method for clonal multiplication of cultivated orchids which are complex hybrids is back bulb propagation it involves separating the oldest pseudo bulbil to force the development buds this process allows at best doubling the plant number every year